Abstract
A cell is an independent functioning unit, and it regulates its input and output based on its own genetic makeup omitting any external transcriptions. Unlike bulk measurement with millions of cells together, which provides an average data, single-cell analysis (SCA) can reflect physiological information of each individual cell and therefore is essential to understand cellular characteristics precisely. For SCA, the delivery of certain molecules into a specific cell type is a challenging task, and it is essential to come up with novel methods to achieve such a goal. Mechanoporation is an emerging and promising method for single cell intracellular delivery, where mechanical energy is used to create transient hydrophilic membrane pores to deliver cargo into the target cell with high transfection efficiency and high cell viability. This method does not depend on the type of cell to be delivered. In this chapter, we have briefly discussed different single-cell mechanoporation devices based on the source of mechanical forces used to create transient membrane pores and intracellular delivery. In addition, the advantages and limitations of each technique are compared, and future prospectsare highlighted.