Abstract
Baculoviruses are a diverse group of insect viruses, among which Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the best characterized. AcMNPV has been widely utilized for recombinant protein production in insect cells, and has captured growing interest as a vector for gene delivery into mammalian cells. This chapter primarily reviews the regulation of AcMNPV gene expression in insect cells and approaches to modulating transgene expression in both insect and mammalian cells. Recent progress and efforts directed towards enhancing the expression levels and extending the expression duration are particularly emphasized. © 2010 by Nova Science Publishers, Inc. All rights reserved.