Abstract
This paper reports a three dimensional circulatory cell perfusion-culture system with difference medium by using proportional cell contact. The system was consisted of two dimensional cell contact chambers and three dimensional cell loading chambers. For cell contact chambers, we used dielectrophoresis pattern method to make three difference contact area for difference proportional communication of HMEC-1 cells and 3T3 cells. For cell loading chambers, we used the PDMS-based micropillars to mimic three dimension lobule-mimetic structures in vitro. Finally, we showed liver functional albumin testing. The albumin secretion of liver pattern chambers with HMEC-1 and 3T3 co-culture communication was 64.8% higher than the albumin secretion of liver chip without cells communication.