Abstract
Fibroblasts are the key components of tumor microenvironment (TME) and activity of these cells induces desmoplastic reactions in the spheroids. In this present work we co-cultured colon cancer cells (HCT-8) and fibroblast cells (NIH3T3) in a micro-well 3D (µ- well 3D) array cell culture chip and produced high throughput, even sized multicellular spheroids. The PDMS and contained 630 round wells, were coated with Pluroninc F-127 self-assembled molecules for rapid single organoids growth. The cross talk between colon cancer cells and fibroblast cells upregulated cancer associated fibroblast (CAF's) markers, which are co-related with increased stiffness and drug resistance in 3D co-cultured spheroids.