Abstract
We have developed a microsystem which mimics the dynamic, three dimensional micro environment in the blood vessel. The capillary-endothelium-mimetic microfluidic chip was used to study the chemotactic behavior of neutrophils by using quantitative approach. The quantitative study shows that at lower flow rates the chemotactic factor dominates over the flow rate increasing the exravasation of neutrophils whereas at higher flow rates the neutrophil extravasation minimizes.