Abstract
This study presents an integrated microfluidic system capable of chemical and mechanical stimuli on human mesenchymal stem cells (hMSCs) for adipogenic differentiation. It is composed of a dilution module for controlling the concentration of medium and membrane structures for tuning the pneumatic force on cells. Experimental data showed that maximum oil droplets were induced under an insulin concentration of 10 |ig/ml. Furthermore, it also revealed that a pressure of 2 psi was optimal for hMSCs growth. The development of the microfluidic system may provide a useful tool for investigating the differentiation of stem cells.