Abstract
We report enzymatic reaction inside extended-nano spaces (10 1 - 10 2 nm) on a chip by using pressure-driven nanofludic control system. We examined the kinetic parameters of enzyme in the extended-nano spaces. The results showed that a first-order rate constant (k cat ) for a nanospace was increased by a factor of about two compared with bulk and micro spaces. This is first observation of nanofluidic-based enzymatic reactions.