Abstract
This study presents a portable device for rapid Influenza A (H1N1) diagnosis utilizing a loop-mediated isothermal amplification (LAMP) approach, which may carry out molecular diagnosis with high sensitivity and specificity. The entire procedure could be automated on a passive microfluidic chip, controlled and monitored via a smart phone. The diagnostic results could be observed colorimetrically and detected by an integrated color sensor. This is the first time that an automated portable device was demonstrated which could perform a molecular diagnosis process including virus purification and nucleic acid amplification to diagnose Influenza A H1N1 virus and may become a promising tool for point-of-care applications.