A protein-analysis chip integrating affinit chromatographic column and isoelectric focusing channel

Yutaka Koyama; Takehiko Kitamori; Kiyohito Shimura

Conference paper

A protein-analysis chip integrating affinit chromatographic column and isoelectric focusing channel

Yutaka Koyama, Takehiko Kitamori and Kiyohito Shimura

Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp.939-941

2006

Appears in keyword about Chemistry

Abstract

Affinity chromatography

Electrophoresis

Isoelectric focusing

Posttranslational modifications

Protein analysis

Chemical Engineering (miscellaneous)

Bioengineering

We report a protein-analysis microchip integrating specific capture by affinity chromatography (AC) and charge-based separation by isoelectric focusing (IEF). Ten fmol each of 4 kinds of peptide isoelectric point (pI) markers in 1 μL of a sample was separated in an IEF channel of the protein-analysis chip. © 2006 Society for Chemistry and Micro-Nano Systems.

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Title: A protein-analysis chip integrating affinit chromatographic column and isoelectric focusing channel
Translated title: A protein-analysis chip integrating affinit chromatographic column and isoelectric focusing channel
Creators - without role: Yutaka Koyama - Department of Applied Chemistry, University of Tokyo
Takehiko Kitamori - Department of Applied Chemistry, University of Tokyo
Kiyohito Shimura - Department of Applied Chemistry, University of Tokyo
Publication Details: Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp.939-941
Identifiers: 9957767804306774
Academic Unit: Institute of NanoEngineering and Microsystems, College of Engineering, National Tsing Hua University
Language: English
Resource Type: Conference paper

A protein-analysis chip integrating affinit chromatographic column and isoelectric focusing channel

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