Abstract
Systematic evolution of ligands by exponential enrichment (SELEX) is a method to screen specific DNA- or RNA-based aptamers by repeated rounds of partition and amplification from random pool of oligonucleotides. The screening products have high binding affinity for the target molecule, which can be an excellent biomarker for diagnosis and therapeutics. This study reports a new continuous microfluidic system to perform SELEX in an automatic mode, which is an excellent tool for fast selection of aptamers. As a demonstration, hemoglomin-specific aptamer was screened. The screened aptamer can be used for fast diagnosis of hemoglobin, which is a crucial indicator for diabetes mellitus.