Abstract
This paper to demonstrate temperature-controlled hydrophobic interaction as a method for capturing and releasing cells. Protein-based cell microarray is a powerful tool used in both clinical diagnostics and fundamental researches. However, the cell of interest after screening could not be released from cell microarray chip for further studies. This research presents the idea of combining antibody-antigen interaction and cell sorting system to improve the specificity. The surface was fabricated by grafting thermo-responsive polymer to PDMS (Polydimethylsiloxane), and the concept of capture and release cell were proved by polystyrene beads. The platform can be optimized and applied to provide further collection of cells of interest from protein-based cell microarray.