Abstract
We present a simple technique to arrange large quantity of living mammalian cells into a self-assembled two-dimensional (2D) monolayer array for rapid targeted cells identification, which is potentially useful for the early detection of circulating tumor cells (CTC). By controlling the concentration of cells suspension, 2 × 10 cells/μl gives the optimized density for 2D cells assembly. With the condition which enable cells align into a 2D monolayer array, the lowest ratio of the targeted cells to the white blood cells this simple method can provide is 1/10,000 by using a fluorescence microscope.