AN INTEGRATED MICROFLUIDIC SYSTEM FOR AUTOMATIC SCREENING DNA APTAMERS FOR HUMAN NEUTROPHIL PEPTIDE 1-3

Hung-Bin Wu; Rishabh Gandotra; Priya Gopinathan; Yi-Da Chung; Huey-Ling You; Feng-Chih Kuo; Mel S. Lee; Gwo-Bin Lee

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Conference paper

AN INTEGRATED MICROFLUIDIC SYSTEM FOR AUTOMATIC SCREENING DNA APTAMERS FOR HUMAN NEUTROPHIL PEPTIDE 1-3

Hung-Bin Wu, Rishabh Gandotra, Priya Gopinathan, Yi-Da Chung, Huey-Ling You, Feng-Chih Kuo, Mel S. Lee and Gwo-Bin Lee

MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp.749-750

2021

Appears in keyword about Chemistry

Abstract

Aptamers

Human neutrophil peptide 1-3

Microfluidics

Periprosthetic joint infection

Systematic evolution of ligands by exponential enrichment

Bioengineering

Chemical Engineering (miscellaneous)

Systematic evolution of ligands by exponential enrichment (SELEX) is a complicated and time-consuming process which requires several iterative rounds to screen aptamers with high specificity and affinity. In this work, a new automatic SELEX platform was developed to select aptamers specific towards an important biomarker, human neutrophil peptide 1-3 (HNP 1-3), for diagnosis of joint infection. Promising aptamer candidates that can be targeted against HNP 1-3 were successfully selected, which could be applied as an artificial antibody.

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Title: AN INTEGRATED MICROFLUIDIC SYSTEM FOR AUTOMATIC SCREENING DNA APTAMERS FOR HUMAN NEUTROPHIL PEPTIDE 1-3
Translated title: AN INTEGRATED MICROFLUIDIC SYSTEM FOR AUTOMATIC SCREENING DNA APTAMERS FOR HUMAN NEUTROPHIL PEPTIDE 1-3
Creators - without role: Hung-Bin Wu - Department of Power Mechanical Engineering , National Tsing Hua University
Rishabh Gandotra - Department of Power Mechanical Engineering , National Tsing Hua University
Priya Gopinathan - Department of Power Mechanical Engineering , National Tsing Hua University
Yi-Da Chung - Department of Power Mechanical Engineering , National Tsing Hua University
Huey-Ling You - Kaohsiung Chang Gung Memorial Hospital
Feng-Chih Kuo - Kaohsiung Chang Gung Memorial Hospital
Mel S. Lee - Kaohsiung Chang Gung Memorial Hospital
Gwo-Bin Lee - Department of Power Mechanical Engineering , National Tsing Hua University
Publication Details: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp.749-750
Identifiers: 9781733419031; 9957768031906774
Academic Unit: Institute of Biomedical Engineering, College of Engineering, National Tsing Hua University
Language: English
Resource Type: Conference paper

AN INTEGRATED MICROFLUIDIC SYSTEM FOR AUTOMATIC SCREENING DNA APTAMERS FOR HUMAN NEUTROPHIL PEPTIDE 1-3

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