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An integrated selex microfluidic system for rapid screening of influenza virus-specific aptamers
Conference paper

An integrated selex microfluidic system for rapid screening of influenza virus-specific aptamers

Hsien-Chih Lai, Chih-Hung Wang, Chen-Hsun Weng, Tong-Miin Liou and Gwo-Bin Lee
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, pp.1402-1404
2012

Abstract

Aptamer Influenza virus Microfluidics SELEX
An integrated microfluidic system was developed to automatically perform systematic evolution of ligands and exponential enrichment (SELEX) process using combinational single-stranded DNA (ssDNA) library for rapid screening of highly specific aptamers for influenza viruses in this study. This integrated microfluidic system consisted of several modules on a single chip. With this system, the specific aptamers were screened successfully with high affinity for influenza viruses after several repeated extraction and amplification runs. Therefore, it may provide a useful platform for specific aptamers screening, which can be a promising biomarker to recognize viruses for fast diagnosis.

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