Abstract
A liquid chromatography using 100-nm scale (extended-nano) channels was developed for single cell analysis. Our method, extended-nano chromatography makes possible an ultrasmall sample (100 aL-1 fL) injection and high efficient separation (∼105-106 plates/m) using nanofluidic channel as separation column. In particular, hydrophilic interaction (HILIC) and reversed-phase chromatography was developed for separation of biomolecules including proteins in a single cell. In addition, an influence of a specific liquid property in extended-nano space was investigated for higher separation efficiency.