Abstract
In this work, we proposed a new design of the glass microchip, where we realized enzyme-linked immunosorbent assay (ELISA). Same as previous the new microchip takes advantages of shallow but wide and long microfluidic channel (depth below 5 urn, width of 1 mm and length of 20 mm) to immobilize a thin layer of an antibody directly on the channel surface (we called it Thin-layered ELISA) without using microbeads. We split the guiding channel into tree to deliver a sample and reagents in a controlled mode without cross-contamination. All microfluidic processes were fully automated via set of programmed valves and pumps. We achieved high antigen capture efficiency and successfully completed a total protein analysis from sample injection till enzymatic reaction detection within 10 mm, with limit of detection for C-reactive protein (CRP) of 0.4 ng/mL. The sample volumes necessary for this assay was 20μL which is smaller than one drop of blood.