Abstract
Bacterial resistance against antimicrobial agents has become a significant issue in modern healthcare. To reduce dosage of broad-spectrum antibiotics, the minimum inhibitory concentration of antibiotics should be determined before administration based on a common practice commonly referred as antimicrobial susceptibility testing (AST). However, conventional AST methods are relatively time-consuming and labor-intensive in screening various antibiotic combinations. To enable faster turnaround time of AST, automation of the entire process could be crucial. This work therefore reported an automatic microfluidic system which could automate four different antibiotic combinations with serial broth dilutions in parallel.