Abstract
Protein chip is a potential tool on advanced disease diagnosis and drug screening, due to the capabilities of parallel process and trace-amount sample detection. The detection of proteins mainly depends on immune recognition, very sensitive to the orientation of immobilized proteins on solid surface. However, due to close packing of immobilized proteins on SAMs, the binding efficiency is usually lower than 10%. This paper proposed a novel way by employing mixed SAMs to generate nano patterns for effective separation of immobilized proteins to allow antibody conjugation from more directions. Testing results by Surface Plasma Resonance (SPR) and Atomic Force Microscope (AFM) investigation demonstrated a almost 3 fold increment on binding efficiency of protein immobilization on mixed SAMs.