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Cell-based microfluidic co-culture system for indication of nitric oxide secrete from Endothelial cells
Conference paper

Cell-based microfluidic co-culture system for indication of nitric oxide secrete from Endothelial cells

Kihoon Jang, Kae Sato, Yo Tanaka, Takahiro Nakajima, Moritoshi Sato and Takehiko Kitamori
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp.1907-1909
2009
Appears in  keyword about Chemistry

Abstract

ATP Co-culture Endothelial cells Microchannel Nitric oxide Piccells Chemical Engineering (miscellaneous) Bioengineering
In this paper, we employed two different types of cells inside the microchannel using photochemical reaction. We have developed a co-culture system which has been expected as one of the most powerful tools for analyzing cell-to-cell interactions in mimicking tissue structures. Our technique was based on combining MPC polymer and photolabile linker that was applied for nitric oxide (NO) analysis which was detected in Piccells release from Endothelial cells (ECs) by ATP stimulation. © 2009 CBMS.

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