Abstract
In this report, we report that we have developed an actuator for fluid actuation for a bio-micropump using thawed frozen cardiomyocytes having a new structure, by using a thin membrane and a cubic block to collect the cardiomyocyte force and communicate it to fluid. We were able to demonstrate fluid motion in a microchannel connected to a diaphragm chamber induced by the synchronously pulsating cardiomyocytes. This new approach reduces the necessity of using animals for the experiments, because frozen cells may be used.