Abstract
In this article we categorize frequency dependent behaviors of HepG2 cell on TiOPc-based optoelectronic dielectrophoresis chip. Previous works of optoelectronic manipulation only focus on positive or negative dielectrophoresis effect of cell manipulation. Our cell behavior calibration fills the blank of frequency domain between positive and negative dielectrophoresis on TiOPc-coated substrate. Besides, the careful investigation of critical frequency would apply to separate and select high and low permeable membrane cells. Finally, utilizing atomic force microscope to measure the TiOPc-coated surface roughness, determining cell viability on TiOPc surface for 168 hrs, and applying negative dielectrophoresis force to arrange cells array provides deeper knowledge for this organic photoconductive material application.