Abstract
Chromatographic separation and detection of nonfluorescent molecules were integrated in a nanochannel. Our method, extended-nano chromatography realized high-performance separation using pressure-driven flow in a 100-nm scale nanochannel. A photothermal detector, differential interference contrast thermal lens microscope (DIC-TLM) was employed for detection of nonfluorescent samples. In particular, two nonfluorescent dyes, Sudan I and Sudan Orange G were separated using hexane and silica nanochannel in normal-phase mode. The detection performance of DIC-TLM was closely examined in terms of time resolution, time response and quantitative performance. Combining extended-nano chromatography with DIC-TLM, separation of nonfluorescent molecules was firstly studied. Copyright © (2011) by the Chemical and Biological Microsystems Society.