Abstract
We here report the new culturing method of vascular cells using a separable microchip whose substrates can be combined and separated reversibly. This separable microchip allowed to culture smooth muscle cells (SMCs) under the medium perfusion, and to subsequently recover the cultured cells from microchannel only by reducing the temperature. This system has a potential to produce micro scale vascular-mimetic tissue consisting of endothelial cells (ECs) and smooth muscle cells (SMCs), and will be useful for the regeneration of various tissues with multilayered complex structures.