Abstract
Clinical tests of liquid and tissue biopsy are critical for cancer diagnosis and prognosis. Therefore, biomarkers capable of be applicable on both tests are of great importance. In this work, on-chip cell culture and a continuous cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX) process were integrated on a single chip for optimizing final aptamer candidates screened from tissue-SELEX that could specifically bind to membrane molecules. Aptamers were successfully screened through this optimized SELEX conditions and could be applied both on the liquid and tissue biopsy in the near future.