Counting single DNA molecule by on-bead rolling circle amplification for quantitative analyses

Reina Ishii; Naoki Sasaki; Kiichi Sato; Kazuma Mawatari; Mats Nilsson; Takehiko Kitamori; Kae Sato

Conference paper

Counting single DNA molecule by on-bead rolling circle amplification for quantitative analyses

Reina Ishii, Naoki Sasaki, Kiichi Sato, Kazuma Mawatari, Mats Nilsson, Takehiko Kitamori and Kae Sato

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Vol.1, pp.70-72

2011

Appears in keyword about Chemistry

Abstract

Beads

DNA

Rolling circle amplification

Control and Systems Engineering

To realize an ultra sensitive determination of specific DNA, we developed and optimized on-bead rolling circle amplification (RCA) method for quantitative analyses. By using agarose beads, detection efficiency increased 100 times compared to the conventional RCA method, and quantitative analyses of 1 μL of samples containing 0.05 - 1.0 nM DNA were realized. Copyright © (2011) by the Chemical and Biological Microsystems Society.

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Title: Counting single DNA molecule by on-bead rolling circle amplification for quantitative analyses
Translated title: Counting single DNA molecule by on-bead rolling circle amplification for quantitative analyses
Creators - without role: Reina Ishii - Japan Women's University
Naoki Sasaki - Japan Women's University
Kiichi Sato - Gunma University
Kazuma Mawatari - The University of Tokyo
Mats Nilsson - Uppsala University
Takehiko Kitamori - The University of Tokyo
Kae Sato - Japan Women's University
Publication Details: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Vol.1, pp.70-72
Identifiers: 9781618395955; 9957775237406774
Academic Unit: Institute of NanoEngineering and Microsystems, College of Engineering, National Tsing Hua University
Language: English
Resource Type: Conference paper

Counting single DNA molecule by on-bead rolling circle amplification for quantitative analyses

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