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DNA extraction from culture medium by using ewod system
Conference paper

DNA extraction from culture medium by using ewod system

Chen-En Chiang, Tzu-Hui Wu, Pei-Shin Jiang, Chien-An Chen and Da-Jenf Yao
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, pp.1616-1617
2019

Abstract

Digital Microfluidics DNA extraction Embryo culture medium Bioengineering Chemical Engineering (miscellaneous)
We have developed a DNA extraction platform by EWOD-based digital microfluidics (DMF), and demonstrated that our platform can extract lower concentration of cfDNA from embryo culture medium of mouse. Compared to traditional methods, our platform used lower volume of reagents on the order of micro-liter or less, and obtained high recovery rate. Therefore, the developed platform can offer the potential bio-applications, such as detection of sample preparations for bacteria, viruses and genetic sequencing, etc.

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