Detection of countable number of molecules by enzyme-linked immunosorbent assay in extended nanochannels

K. Shirai; R. Ohta; K. Mawatari; H. Shimizu; T. Kitamori

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Conference paper

Detection of countable number of molecules by enzyme-linked immunosorbent assay in extended nanochannels

K. Shirai, R. Ohta, K. Mawatari, H. Shimizu and T. Kitamori

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, pp.817-819

2014

Appears in keyword about Chemistry

Abstract

Countable molecules

DIC-TLM

ELISA

Extended nanospace

Control and Systems Engineering

We developed a nanofluidics-based enzyme-linked immunosorbent assay (ELISA) device and realized quantification of countable number of molecules by combining enzymatic amplification reaction and ultrasensitive detection with a differential interference contrast thermal lens microscope (DIC-TLM). Our method will contribute to single cell analysis field, in particular for ultrasensitive protein counting.

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Title: Detection of countable number of molecules by enzyme-linked immunosorbent assay in extended nanochannels
Translated title: Detection of countable number of molecules by enzyme-linked immunosorbent assay in extended nanochannels
Creators - without role: K. Shirai - Department of Applied Chemistry, School of Engineering, University of Tokyo
R. Ohta - Department of Applied Chemistry, School of Engineering, University of Tokyo
K. Mawatari - Department of Applied Chemistry, School of Engineering, University of Tokyo
H. Shimizu - Department of Applied Chemistry, School of Engineering, University of Tokyo
T. Kitamori - Department of Applied Chemistry, School of Engineering, University of Tokyo
Identifiers: 9780979806476; 9957771309406774
Academic Unit: Institute of NanoEngineering and Microsystems, College of Engineering, National Tsing Hua University
Language: English
Resource Type: Conference paper
Publication Details: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, pp.817-819

Detection of countable number of molecules by enzyme-linked immunosorbent assay in extended nanochannels

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