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Development of extended-nano femtolitter mess-pipette in living single cell analysis device
Conference paper

Development of extended-nano femtolitter mess-pipette in living single cell analysis device

Tatsuro Nakao, Yutaka Kazoe, Kyojiro Morikawa, Ling Lin, Ayumi Yoshizaki, Kazuma Mawatari and Takehiko Kitamori
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, pp.930-931
2017

Abstract

Extended-Nano Space;Femto Litter;Pipette;Single Cell Chemical Engineering (miscellaneous) Bioengineering

Here we report a development of fL (10 L) mess-pipette (volumetric pipette) utilizing precise fabrication of 100-nm structure on fused silica and hydrophobic Laplace valve. During verification of fL volumetric pipette, we discovered that drying of sample is the key issue on fL liquid handling in gas phase. By quick application of pressure (~ms), we minimized the effect of drying and 11 fL of water was successfully isolated and transported without loss. This study will greatly contribute to analytical chemistry by providing a novel method of volumetry and manipulation of fL liquid.

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