Abstract
In this study, an implanted self-perfusion insulin delivery device composed of cardiomyocytes and Lagerhans islets for diabetes patient is proposed. Firstly, cardiomyocytes were cultured in closed space on polydimethlsiloxane (PDMS) microchip in the proper condition (-incubation time to adhere 2.0 h, medium perfusion rate 1.0 μm/min) and confluent state was achieved. Cardiomyocytes were then cultured and about 11 % of cells adhered in a PDMS hollow sphere. Secondly, co-culture of cardiomyocytes and Langerhans islets were achieved and insulin production was confirmed in a micro titer plate. Thus we established the fundamental technology of implanted insulin delivery device.