Abstract
Sepsis is caused by bacterial infection and excessive immune response. Since bacteria are ingested into white blood cells, pathogen can be identified by analyzing leukocytes in whole blood and detecting bacterial DNA. We detected mitochondrial DNA, bacterial derived organelle, included in leukocytes, non-adherent cells, to diagnose sepsis. We applied in-situ rolling circle amplification (RCA) to genotyping, and realized in-situ RCA on microchip for the first time. © 2009 CBMS.