Abstract
In microfluidics, there are two demands for immunoassay capability, one is detection with a small amount of sample volume rapidly for point-of-care-testing, and another is detection of low-abundant protein for determination of medical treatment even if it takes long time. The former method has been already established by our group. In this research, for the latter demand, new analysis method have been investigated for the first time. We have tried to concentrate target protein between the beads in microchannel by flowing large amount of sample. As a result, quantification of anti-MDA-5 antibody, contained low abundant in patient serum, was achieved.