Abstract
Thin-layered fluidics has realized high efficiency capture of low concentration protein, rapid analysis, and high sensitive detection in between the size of micro and extended-nano fluidics. In this research, for the purpose of practical repeatable use of the ELISA devices, we have investigated washing method, storage method, storage stability, and realized more than 10 times use and 120 days storage. The advantage of repeated measurement is that the patient sample can be measured continuously in the same channel after calibration of standard sample.