Development of specific single-cell gene analysis system on a microchip

Jun Wakabayashi; Yo Tanaka; Kae Sato; Kazuma Mawatari; Yuki Tanaka; Mats Nilsson; Takehiko Kitamori

Conference paper

Development of specific single-cell gene analysis system on a microchip

Jun Wakabayashi, Yo Tanaka, Kae Sato, Kazuma Mawatari, Yuki Tanaka, Mats Nilsson and Takehiko Kitamori

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Vol.2, pp.764-766

2010

Appears in keyword about Chemistry

Abstract

Gene Detection

Microchip

Rolling Circle Amplification

Single-Cell

Control and Systems Engineering

For detailed clarification of the molecular mechanism of cancer or other diseases, gene detection system at single cell and single molecule level is required. In order to realize that system, this paper reports both quantitative and qualitative analysis method of small amount of DNA by in situ padlock probe/rolling circle amplification (RCA) on a mi-crofluidic device.

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Title: Development of specific single-cell gene analysis system on a microchip
Translated title: Development of specific single-cell gene analysis system on a microchip
Creators - without role: Jun Wakabayashi - The University of Tokyo
Yo Tanaka - The University of Tokyo
Kae Sato - JST , CREST
Kazuma Mawatari - The University of Tokyo
Yuki Tanaka - Uppsala University
Mats Nilsson - Uppsala University
Takehiko Kitamori - The University of Tokyo
Publication Details: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Vol.2, pp.764-766
Identifiers: 9781618390622; 9957777115506774
Academic Unit: Institute of NanoEngineering and Microsystems, College of Engineering, National Tsing Hua University
Language: English
Resource Type: Conference paper

Development of specific single-cell gene analysis system on a microchip

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