Abstract
Statement of Purpose: Cancer stem cells (CSC) are a sub-population of cells and are recently proposed to be the cancer initiating cells responsible for tumor formation, recurrence, metastasis and contribute to cancer resistance. Chemotherapeutic drugs are mostly effective on normal cancer cells leaving CSCs mostly unaffected after treatment. Their quiescence nature makes them prime suspects to be responsible for resistance to anti-proliferative chemotherapy. Our previous study has used hyaluronic acid (HA) based multilayer film system to select liver cancer stem cell colonies. Several studies have demonstrated that low intensity ultrasound can induce stem cell differentiation and also can improve the anti-cancer activity of chemotherapeutic drugs. The goal of this study is to facilitate possibility of differentiation therapy of liver CSCs by using low intensity ultrasound.