Logo image
Efficient and rapid capture of DNA utilizing 100-1000 NM scale channel with DNA patterning
Conference paper

Efficient and rapid capture of DNA utilizing 100-1000 NM scale channel with DNA patterning

T. Nakao, K. Mawatari, H. Shimizu and T. Kitamori
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp.858-860
2015

Abstract

DNA Efficient capture Hybridization Microfluidics Quantification Control and Systems Engineering
This paper reports the novel method of efficient and rapid capture of DNA utilizing 10 -10 nm-sized glass channel with DNA patterning. In this paper, capture DNA was site-specifically (3 mm × 2 μm) grafted on a wall of channels and utilized as a DNA capture region in the glass chip (7 cm × 3cm). We utilized the methodology of 100% molecule capture by designing the channel considering the theory of Brownian motion. The capturing performance was evaluated by monitoring the change of fluorescence intensity while introducing the fluorescence-labeled target DNA. Capture with almost 100% efficiency and ∼10 min rapidity was achieved. This work is contributable as a novel platform for DNA analysis.

Metrics

1 Record Views

Details

Logo image