Abstract
An active electrokinetically-driven micro-mixer integrated with an on-chop temperature control system is studied and applied for DNA amplification process. In this DNA amplification process, the cell lysis is first performed in a micro cell lysis reactor. Extracted DNA, primers and reagents are then driven electroosmoticaly into a mixing region where they are mixed by the active micro-mixer. The homogeneous mixture is then thermally cycled in a micro-PCR(polymerase chain reaction) chamber to perform DNA amplification. the novel microfluidic system, which facilitates cell lysis, sample driving/mixing, and DNA amplfication, could provides a significant contribution to ongoning efforts to miniaturize bio-analysis systems.