Abstract
Intensity interrogation of SPR biosensor owns high sensitivity, and is generally used as SPR microscopy due to the optical intensity variation. Therefore, it is substantial to improve its sensitivity to have a better sensing ability and image quality. In this paper, we discussed numerically and experimentally the influence of sensitivity by metal thickness, and provide a design rule of manifesting optimized thickness to maximize sensitivity in intensity interrogation. © 2009 Copyright SPIE - The International Society for Optical Engineering.