Abstract
Single DNA detection becomes a focus in genomics, diagnosis, and medical genetics. Information of each single molecule in each single cell is necessary in medical genetics diagnoses. The Rolling Circle Amplification (RCA) is one of the popular methods to detect single DNA, however it is difficult to be applied to ultra small volume samples such as single cells. Here, we demonstrated patterning and detection of single molecule DNA in extended-nano (10-1000 nm) channels for small volume sample analysis.