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Extraordinary Acoustic Raman Spectroscopy of PR65
Conference paper

Extraordinary Acoustic Raman Spectroscopy of PR65

Elham Babaei, Ghazal Hajisalem, Shohei Iwamoto, Burak Kaynak, Pemra Doruker, Mohsin M. Naqvi, Janet Kumita, Feng-Yu Wang, Jhih-Hong Cheng, Che-Min Wu, …
2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021
2021
DOI: https://doi.org/10.1109/NMDC50713.2021.9677469

Abstract

Acoustic Raman spectroscopy DNH optical tweezer PR65 protein trapping Electronic Optical and Magnetic Materials Metals and Alloys Instrumentation Hardware and Architecture Electrical and Electronic Engineering Mechanical Engineering
Using double nanohole (DNH) optical tweezer setup and applying two lasers, we trapped and excited acoustic vibration modes of the PR65 subunit within the protein phosphatase 2A (PP2A). Our experiment shows that the vibrational modes are at frequencies of 24.26 GHz, 32.80 GHz, 48.47 GHz, 57.97 GHz which matches to the normal mode analysis results. This technique is single molecule and label free compared to typical methods used for characterization of biomolecules. Characterizing PR65 can be used as a paradigm for understanding the structural regulation of many repeat proteins.

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