Abstract
This paper demonstrate protein crystallization optimization using hydrogel-digitized Microfluidic System. This technique is incorporated with Feedback-System-Control (FSC) algorithm for fast and easy screening of chemical conditions required for obtaining high quality and large protein crystals. We have demonstrated the efficacy of our technique by crystallizing Lysozyme protein. Large and high quality protein crystals could be obtained by conducting much fewer crystallization trials in parallel from a pool of thousands of possible trials; thus our methodology allows for thorough screening of crystallization conditions without the need for elaborate and expensive robotic solutions.