Abstract
The requirement for single cell analysis has increased because of cell heterogeneity in cell biology and medical fields. Micro/Nanofluidic chips will be a powerful tool to realize single cell analysis. In order to develop total integration living single cell analysis, highly precise fluidic control is one of the key tools. Here, we developed Laplace nano-valve using new surface patterning for fL sampling control and transport. This new method allows ultra small volume sample (fL) controlling essential for single cell and single-molecule analysis and contributes to cell biology and medical diagnosis.