Abstract
Recently, the requirement for living single cell analysis is rapidly increasing because of the recognition of the cell heterogeneity in cell biology and medical fields. However, single cell volume is ∼pL and too small to analyze by conventional method. Based on this consideration, we reached an idea to realize living single cell analysis by developing fL sampling from the single cell and analyzing the proteins/metabolites in the extended-nano channel. In this study, we reported fL sampling method from living single cell by developing an extended-nano/micro interface utilizing cell fusion with lipid-modified extended-nano channel. This new sampling method allows ultrasensitive analysis of living single cells and contributes to cell biology and tissue engineering.