Abstract
This paper reports single DNA molecule analysis in single cells using a microfluidic platform. Single-nucleotide polymorphisms (SNPs) in individual mitochondrial DNA molecules were analyzed using padlock probes, made visible by rolling-circle amplification of the circularized padlock probes [1]. By combining microfluidcs with padlock probes, individual cells can be manipulated and investigated with singlemolecule sensitivity. The microfluidic format also gives us the possibility to subject a fraction of the cells on the device to specific treatment and to observe the response. © 2006 Society for Chemistry and Micro-Nano Systems.