Gold-nanoparticle-enhanced IGG immunological detection by in-situ Fabry-Perot sensor

Yuan-Tai Tseng; Shu-Ting Jhuang; Chung-Shi Yang; Fan-Gang Tseng; IEEE

doi:10.1109/memsys.2007.4432985

Gold-nanoparticle-enhanced IGG immunological detection by in-situ Fabry-Perot sensor

Yuan-Tai Tseng, Shu-Ting Jhuang, Chung-Shi Yang Fan-Gang Tseng

PROCEEDINGS OF THE IEEE TWENTIETH ANNUAL INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, VOLS 1 AND 2, p.103

Proceedings IEEE Micro Electro Mechanical Systems, IEEE

01/01/2007

: https://doi.org/10.1109/memsys.2007.4432985

: WOS:000255867800026

Engineering, Electrical & Electronic

Nanoscience & Nanotechnology

Science & Technology

Science & Technology - Other Topics

Engineering

Technology

This paper proposes a needle-type biosensor for immunological application, which combines the abilities of real-time monitoring, in-situ measurement and high sensitivity, by adopting gold-nanoparticles (GNPs) coated with antibody to enhance the signal of Fabry-Perot (F-P) interferometry in an optic fiber sensing system. Strong reflection induced by larger index mismatch and longer optical path from GNPs-protein conjugation are suggested to attribute most to the signal enhancement in F-P interference shift. The experiments carry out the monitoring of immuno-sensing in real-time by the employment of rabbit IgG conjugate to GNPs-anti-rabbit IgG And the detection limit was demonstrated to approach 0.17 nM (similar to 25.5 ng/ml), which was three orders of magnitude better than those of the traditional fiber-based F-P biosensors. Besides, the reproducibility of the sensor has been tested through at least three cycles of detection/surface-regeneration process and demonstrated a reasonable consistency. The relationship between the concentrations of GNPs-antibody and F-P spectrum shift has also been characterized. The time-constant of this sensor to complete one cycle of biomolecule-detection and surface-regeneration can be as low as 4 minutes.

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: Gold-nanoparticle-enhanced IGG immunological detection by in-situ Fabry-Perot sensor
: Yuan-Tai Tseng - Natl Tsing Hua Univ, Inst MicroElectroMech Syst, Hsinchu 300, Taiwan
Shu-Ting Jhuang - Natl Tsing Hua Univ, Dept Engn & Sys Sci, Hsinchu 300, Taiwan
Chung-Shi Yang - Ctr Nanomed Res, Natl Hlth Res Inst, Zhunan 307, Taiwan
Fan-Gang Tseng - Natl Tsing Hua Univ, Inst MicroElectroMech Syst, Hsinchu 300, Taiwan
IEEE
: Proceedings IEEE Micro Electro Mechanical Systems
: IEEE
: 2
: VUST project (Veterans General Hospital and University System of Taiwan), NHRI (National Health Research Institutes, Taiwan).; National Health Research Institutes - Taiwan
: Conference paper
: 01/01/2007
: PROCEEDINGS OF THE IEEE TWENTIETH ANNUAL INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, VOLS 1 AND 2, p.103
: English