Abstract
Recently, sperm sorting by microfluidic chips shows an promising efficiency. Traditional devices are designed to collect top-tier sperms which swim in upstream. The collected sperm numbers and total volume are 1~10*10 5 sperm cells and 1~10 μL, respectively, which is good for mankind IVF. However, for pig's artificial insemination, it requires 2*10 9 sperm cells and 200ml semen volume. Therefore, a high-throughput microfluidic device is proposed. The concept of this design is to sweep-out the weak sperms from a chamber, and we could collect 5*10 7 motile sperm cells (80% motility) with total volume of 1 ml within 45 minutes.