Abstract
This paper introduces a hand controllable single cell trapping and incubation network passively employing surface tension force to gently manipulate individual cell for single-cell-based research. The operations of bath registration of single cell array, precise parallel dosage for individual channel in nl resolution, and the replacement of incubation solution repeatedly have been successfully demonstrated in this work. This simple system can be operated by hands with pipette and polymer absorbent without the need of precise active control means. © 2006 Society for Chemistry and Micro-Nano Systems.