Abstract
Early diagnosis of bacterial infection is the key for improved survival by prompt and adequate antibiotic in the first few hours (2-3 hours) of infection. In this work, we demonstrate a possibility to rapid detection several pathogenic bacteria from blood plasma in 30 mins. Using modified gold nanoparticle enlarge Surface Enhanced Raman Scattering (SERS) on a High-Density 3D nanostructured Au/Si-pillar arrays platform (10-12 array/μm 2 ) combined with AC electroosmoic (ACEO) trapping. ACEO and corresponding electrostatic force contributed to the binding affinity and reduce the diffusion time. The limit of detection could be lowered to 1-5 cells/ml detected by SERS measurements. The dynamic range of the sensor system ranged from 10 2 -10 6 cells/mL. The results showed that the portable biochip system has great potential as a device for single-particle or possibly even single-organism detection.