Abstract
This study propose a microfluidic device to selectively concentrate bacteria and rule out blood cells in a three dimensional manner through the synergic effects of AC electroosmotic flow (ACEOF) and dielectrophoresis (DEP). This significant concentration not only reduce background noises from blood but also increased 3 orders of magnitude in signals. Gram-negative (Staphylococcus aureus) and gram-positive (Esherichia coli) strains were identified via Raman unique fingerprints with a detection limit as low as 10 2 cfu/ml. Moreover, a rapid antibiotic susceptibility test (AST) were operated in two hours, which shows high potential to help mitigate the challenge of drug resistance in clinical microbiology.