Abstract
In this paper we successfully fabricated a microchip with wide and long (800 nm hydrodynamic diameter and 10 mm length) extended-nano channel and realized the reversed phase liquid chromatography separation of amino acids after octadecylsilylation (ODS). The separation performance characteristics were obtained. The permeability of this nanochannel (K= 47×10 m ) is comparable with that of monolithic column, also it exhibit very low separation impedance (E = 13). Plate number counts were 4000 and plate height was 2.5 um. Due to its size this channel can be applied for macromolecules separation (proteomics).