Abstract
This paper reports the reversed phase HPLC separation in the extended-nanospace (101 to 103 nm space). Single cell time-resolved analysis requires a novel analytical platform which has much smaller volume (fL to aL) than a cell (pL). Our extended-nanospace chromatography meets the requirements and has the potential to analyze the intracellular contents. In this work we present the reversed-phase modification of nanochannel with ODS and its application for amino acids separation.